WebSplenocytes from a Lewis rat were stained with PE-Cy™7 Mouse Anti-Rat CD45 antibody (Cat. No. 561588; solid line histogram) or with a PE-Cy™7 Mouse IgG1, κ Isotype Control (Cat. No. 557872; dashed line histogram). The fluorescence histograms were derived from events with the forward and side light-scatter characteristics of viable splenocytes. WebJan 5, 2012 · CD45/side scatter (SS) gating is the most commonly used approach to blast isolation. This technique is imprecise, however, as many cell populations other than blasts contaminate the “blast gate” (BG), including granulocytes, monocytes, basophils, hematogones, erythroid precursors, and lymphocytes. 1 Rigidly using a CD45/SS gate to …
ALX804601C10_价格-品牌-详情介绍_丁香通
WebNov 16, 2006 · It has been shown that expression of CD45 on myeloma cells has prognostic value and that the CD45 positive cells contain the proliferative fraction of the myeloma cells. We have also demonstrated that the CD45+ population has a higher apoptotic rate compared to CD45− cells. Given the hypothesis that CD45+ cells represent myeloma … WebThe CD45 molecules are single chain integral membrane proteins comprising at least five isoforms ranging from 180 to 220 kDa. They are generated by alternative splicing … jowett brothers
Purified Mouse Anti-CD45 - BD Biosciences
WebDescription: The 104 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.2-expressing mouse strains. The strains that express CD45.2 include the most commonly used mouse strains C57BL/6, BALB/c, C58, DBA/1, DBA/2, C3H/He, CBA, 129, A and AKR. CD45.2 is expressed by all leukocytes in … WebAug 12, 2009 · Flow Cytometry. After lyses of erythrocytes, a total of 1 × 10 6 cells were stained for 15 min at room temperature in the dark with 10 µL of phycoerythrin-cyanin 5.1-conjugated mouse anti-human CD45 monoclonal antibody (Immunotech, France) and R-phycoerythrin (PE)-conjugated mouse anti-human CD56 monoclonal antibody … how to make a compactor create